Position：T7-tag（MG11）Mouse Monoclonal Antibody
| Download attachment:PDF T7 label mouse monoclonal antibody manual
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| T7-tag（MG11）Mouse Monoclonal Antibody |
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| Product number DIB1014 DIB1014L | |
| specification 100ul 1ml | |
| Product Name: Anti-T7 Tag Mouse Monoclonal Antibody | |
| Antibody type: mouse IgG1 | |
| Clone number: MG11 | |
| Concentration: 1mg/ml | |
| Immunogen: Synthesize MASMTGGQQMG coupled with KLH as antigen. | |
| Product description: T7 tag protein consists of 11 amino acid peptides, derived from T7 phage gene 10.T7 tags exist in a variety of expression vectors including PET expression system.The company's T7 antibody is a highly purified monoclonal antibody that can highly specifically recognize the HA tag sequence at the N-terminal, C-terminal and middle positions of the fusion protein. | |
| Recommended dilution: Western blot (WB): 1:1000—1:10000 | |
| Immunofluorescence (IF): 1:100—1:1000 | |
| Immunoprecipitation (IP): 1:100—1:1000 | |
| Solution composition: 0.01M PBS (PH7.4), 0.1% BSA, 0.02% sodium azide, 50% glycerol. | |
| Storage conditions: Store at -20°C. | |
| Validity period: 12 months. | |
| Matters needing attention: 1. Please choose to use anti-mouse secondary antibody, such as goat anti-mouse IgG (H+L)-HRP (DIB3006). |
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| 2. Please determine the best antibody dilution according to the experiment. | |
| 3. This product is only used for scientific research and cannot be used for clinical diagnosis. | |
| Relevant data: | |
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Use T7 tag fusion protein to detect T7-tag antibody (DIB1014 ), the antibody is diluted 1:5000, and the amount of protein loaded per well is 100ng (1), 50ng (2), 25ng (3), 12.5ng (4), 6ng(5), 3ng(6), 1.5ng(7). |