| Download attachment:PDF GFP label mouse monoclonal antibody manual |
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| GFP-tag（ME11）Mouse Monoclonal Antibody |
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| Product number DIB1013 DIB1013L | |
| specification 100ul 1ml | |
| Product Name: Anti-GFP Tag Mouse Monoclonal Antibody | |
| Antibody type: mouse IgG1 | |
| Clone number: ME11 | |
| Concentration: 1mg/ml | |
| Immunogen: GFP full-length protein as antigen. | |
| Product description: GFP (Green Fluorescent Protein) is a luminescent protein found in jellyfish. It is composed of 238 amino acids and has a molecular weight of 26KD.The green fluorescent protein gene is very stable after being transferred into host cells and has no effect on the physiology of most hosts. It is a commonly used reporter gene.The company's GFP antibody is a highly purified monoclonal antibody that can highly specifically recognize the GFP sequence in the fusion protein. | |
| Recommended dilution: Western blot (WB): 1:1000—1:10000 | |
| Immunofluorescence (IF): 1:100—1:1000 | |
| Immunoprecipitation (IP): 1:100—1:1000 | |
| Solution composition: 0.01M PBS (PH7.4), 0.1% BSA, 0.02% sodium azide, 50% glycerol. | |
| Storage conditions: Store at -20°C. | |
| Validity period: 12 months. | |
| Matters needing attention: 1. Please choose to use anti-mouse secondary antibody, such as goat anti-mouse IgG (H+L)-HRP (DIB3006). |
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| 2. Please determine the best antibody dilution according to the experiment. | |
| 3. This product is only used for scientific research and cannot be used for clinical diagnosis. | |
| Relevant data: | |
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Use GFP protein to detect GFP-tag antibody (DIB1013 ), and the amount of protein loaded per hole is 100ng.The antibody was diluted 1:5000 (1), 1:10000 (2), and 1:20000 (3). |
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Detect the GFP-tag antibody (DIB1013 ) with GFP-containing protein, the antibody is diluted 1:5000, and the amount of protein loaded per well is 20ng (1), 10ng (2), 5ng (3), 2.5ng (4), 1.2 ng(5), 0.6ng(6). |