| Download attachment:PDF V5 label mouse monoclonal antibody manual
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| V5-tag（MD6）Mouse Monoclonal Antibody |
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| Product number DIB1011 DIB1011L | |
| specification 100ul 1ml | |
| Product Name: Anti-V5 Tag Mouse Monoclonal Antibody | |
| Antibody type: mouse IgG1 | |
| Clone number: MD6 | |
| Concentration: 1mg/ml | |
| Immunogen: Synthesize GKPIPNPLLGLDST coupled with KLH as antigen. | |
| Product description: V5 tag is a sequence of 14 amino acid residues isolated from the P and V proteins of simian virus 5 (SV5).The company's V5 antibody is a highly purified monoclonal antibody that can highly specifically recognize the V5 sequence at the N-terminal, C-terminal and middle positions of the fusion protein. | |
| Recommended dilution: Western blot (WB): 1:1000—1:10000 | |
| Immunofluorescence (IF): 1:100—1:1000 | |
| Immunoprecipitation (IP): 1:100—1:1000 | |
| Solution composition: 0.01M PBS (PH7.4), 0.1% BSA, 0.02% sodium azide, 50% glycerol. | |
| Storage conditions: Store at -20°C. | |
| Validity period: 12 months. | |
| Matters needing attention: 1. Please choose to use anti-mouse secondary antibody, such as goat anti-mouse IgG (H+L)-HRP (DIB3006). |
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| 2. Please determine the best antibody dilution according to the experiment. | |
| 3. This product is only used for scientific research and cannot be used for clinical diagnosis. | |
| Relevant data: | |
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The His-tag antibody (DIB1011 ) was detected with the fusion protein containing the V5 tag. The sample volume per well was 100ng, and the antibody was diluted 1:5000 (1), 1:10000 (2) and 1:20000 (3). |
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The HA-tag antibody (DIB1011) was detected with the fusion protein containing V5 tag, the antibody was diluted 1:5000, and the protein loading amount per hole was 50ng (1), 25ng (2), 12.5ng (3), 6ng (4 ), 3ng(5). |
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