Position：GST-tag（MB10）Mouse Monoclonal Antibody
| Download attachment:PDF GST label mouse monoclonal antibody manual
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| GST-tag（MB10）Mouse Monoclonal Antibody |
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| Product number DIB1010 DIB1010L | |
| specification 100ul 1ml | |
| Product Name: Anti-GST Tag Mouse Monoclonal Antibody | |
| Antibody type: mouse IgG1 | |
| Clone number: MB10 | |
| Concentration: 1mg/ml | |
| Immunogen: Full-length GST protein as antigen. | |
| Product description: GST (glutathione sulfhydryl transferase) tag protein itself is a transferase that plays an important role in the detoxification process, with a molecular weight of 26KD.The advantages of the GST tag include: improving the expression of the fusion protein, increasing the solubility of the fusion protein, and convenient purification. It can be directly purified with glutathione agarose gel.The company's GST antibody is a highly purified monoclonal antibody that can highly specifically bind to the GST sequence in the fusion protein. | |
| Recommended dilution: Western blot (WB): 1:1000—1:10000 | |
| Immunofluorescence (IF): 1:100—1:1000 | |
| Immunoprecipitation (IP): 1:100—1:1000 | |
| Solution composition: 0.01M PBS (PH7.4), 0.1% BSA, 0.02% sodium azide, 50% glycerol. | |
| Storage conditions: Store at -20°C. | |
| Validity period: 12 months. | |
| Matters needing attention: 1. Please choose to use anti-mouse secondary antibody, such as goat anti-mouse IgG (H+L)-HRP (DIB3006). |
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| 2. Please determine the best antibody dilution according to the experiment. | |
| 3. This product is only used for scientific research and cannot be used for clinical diagnosis. | |
| Relevant data: | |
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The GST-tag antibody (DIB1010) was detected with a fusion protein containing a GST tag, and the amount of protein loaded per well was 20ng.The antibody was diluted 1:5000 (1), 1:10000 (2), 1:20000 (3). |
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The GST-tag antibody (DIB1010) was detected with a fusion protein containing a GST tag. The antibody was diluted 1:5000, and the protein loading amount per hole was 60ng (1), 30ng (2), 15ng (3), 7.5ng (4). ), 3.75ng(5), 1.8ng(6), 0.9ng(7), 0.45ng(8). |