Position：Myc-tag（ME8）Mouse Monoclonal Antibody
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| Myc-tag（ME8）Mouse Monoclonal Antibody |
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| Product number DIB1008 DIB1008L | |
| specification 100ul 1ml | |
| Product Name: Anti-Myc Tag Mouse Monoclonal Antibody | |
| Antibody type: mouse IgG1 | |
| Clone number: ME8 | |
| Concentration: 1mg/ml | |
| Immunogen: Artificially synthesized EQKLISEEDL peptide coupled with KLH as antigen. | |
| Product description: Myc tag is derived from human c-Myc 410-419 peptide.The Myc tag can be placed on the C-terminal or N-terminal, but the low pH elution conditions of Myc recombinant protein tend to reduce the viability of the protein, so the Myc tag system is widely used for detection but is rarely used for purification.The Myc tag has been successfully used in WB hybridization technology, IP immunoprecipitation and flow cytometry.The company's Myc antibody is a highly purified monoclonal antibody that can highly specifically recognize the Myc sequence at the N-terminal, C-terminal and middle positions of the fusion protein. | |
| Recommended dilution: Western blot (WB): 1:1000—1:10000 | |
| Immunofluorescence (IF): 1:100—1:1000 | |
| Immunoprecipitation (IP): 1:100—1:1000 | |
| Solution composition: 0.01M PBS (PH7.4), 0.1% BSA, 0.02% sodium azide, 50% glycerol. | |
| Storage conditions: Store at -20°C. | |
| Validity period: 12 months. | |
| Matters needing attention: 1. Please choose to use anti-mouse secondary antibody, such as goat anti-mouse IgG (H+L)-HRP (DIB3006). |
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| 2. Please determine the best antibody dilution according to the experiment. | |
| 3. This product is only used for scientific research and cannot be used for clinical diagnosis. | |
| Relevant data: | |
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Detection by fusion protein containing Myc tag Myc-tag antibody (DIB1008), egg per well The amount of white sample is 20ng. Antibody ratio is 1:5000 （1），1:10000（2），1:20000(3)， Dilute with 1:40000(4) and 1:80000(5). |
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Detection of Myc-tag antibody by fusion protein containing myc tag (DIB1008), the antibody was diluted at 1:5000, on each well of protein Samples were 50ng(1), 25ng(2), 12.5ng(3) respectively. 6ng（4），3ng（5），1.5ng(6)。 |