Position：Flag-tag（MA4）Mouse Monoclonal Antibody
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Flag label mouse monoclonal antibody instructions
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| Flag-tag（MA4）Mouse Monoclonal Antibody |
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| Product number DIB1007 DIB1007L | |
| specification 100ul 1ml | |
| Product Name: Anti-Flag Tag Mouse Monoclonal Antibody | |
| Antibody source: mouse IgG1 | |
| Clone number: MA4 | |
| Concentration: 1mg/ml | |
| Immunogen: Artificially synthesized DYKDDDDK coupled with KLH as an antigen. | |
| Product description: Flag tag protein is composed of a hydrophilic polypeptide encoding 8 amino acids, and is widely used in the expression detection and purification of fusion proteins.The advantages of Flag tags include: small molecular weight, generally does not affect the function of the target protein; Flag antibody affinity purification can be used to obtain active target protein.The company's Flag antibody is a highly purified monoclonal antibody that can highly specifically recognize the N-terminal, C-terminal, and mid-position Flag sequences in the fusion protein. | |
| Recommended dilution: Western blot (WB): 1:1000—1:10000 | |
| Immunofluorescence (IF): 1:100—1:1000 | |
| Immunoprecipitation (IP): 1:100—1:1000 | |
| Solution composition: 0.01M PBS (PH7.4), 0.1% BSA, 0.02% sodium azide, 50% glycerol. | |
| Storage conditions: Store at -20°C. | |
| Validity period: 12 months. | |
| Matters needing attention: 1. Please choose to use anti-mouse secondary antibody, such as goat anti-mouse IgG (H+L)-HRP (DIB3006). |
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| 2. Please determine the best antibody dilution according to the experiment. | |
| 3. This product is only used for scientific research and cannot be used for clinical diagnosis. | |
| Related Information: | |
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Testing with fused egg white with Flag label The Flag-tag antibody (DIB1007) was applied to each well The amount is 20ng, and the antibody ratio is 1:5000(1) 1:10000(2) and 1:20000(3) dilution. |
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Testing with fused egg white with Flag label The Flag-tag antibody (DIB1007) is Dilute at 1:5000, and the sample loading amount of each hole is 10ng respectively （1），5ng（2），2.5ng（3），1.25ng（4）。 |