Position：His-tag (MC10) Mouse Monoclonal Antibody
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His Tag Mouse Monoclonal Antibody Instructions
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| His-tag (MC10) Mouse Monoclonal Antibody |
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| Product number DIB1006 DIB1006L | |
| specification 100ul 1ml | |
| Product Name: Anti-His Tag Mouse Monoclonal Antibody | |
| Antibody source: mouse IgG1 | |
| Clone number: MC10 | |
| Concentration: 1mg/ml | |
| Immunogen: Artificially synthesized HHHHHH coupled with KLH as an antigen. | |
| Product description: His refers to a fusion tag composed of six histidines, which is widely used in the expression detection and purification of fusion proteins.The advantages of the His tag are: His tag has a small molecular weight and generally does not affect the function of the target protein; His histidine has a strong adsorption force with solid nickel, and the His tag protein can be purified with a Ni column even under denaturing conditions .Our company's His antibody is a highly purified monoclonal antibody that can highly specifically recognize the His sequence at the N-terminal and C-terminal of the fusion protein. | |
| Recommended dilution: Western blot (WB): 1:1000-1:10000 | |
| Immunofluorescence (IF): 1:100-1:1000 | |
| Immunoprecipitation (IP): 1:100-1:1000 | |
| Solution composition: 0.01M PBS (PH7.4), 0.1% BSA, 0.02% sodium azide, 50% glycerol. | |
| Storage conditions: Store at -20°C. | |
| Validity period: 12 months. | |
| Matters needing attention: 1. Please choose to use anti-mouse secondary antibody, such as goat anti-mouse IgG (H+L)-HRP (DIB1006). |
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| 2. Please determine the best antibody dilution according to the experiment. | |
| 3. This product is limited to scientific research and cannot be used for clinical diagnosis. | |
| Related Information: | |
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The fusion egg white containing His label was used to detect His-tag antibody (DIB1006), and the amount of sample per well was 100ng. the antibody was diluted with 1:5000 (1) 1:10000 (2) and 1:20000 (3) respectively. |
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Use the His-tag fusion protein to detect His-tag antibody (DIB1006), the antibody is diluted 1:5000, and the loading amount per well is 20ng (1), 10ng (2), 5ng (3), 2.5ng (4) , 1.25ng (5). |