DMEM/High Glucose Medium, also known as DMEM (Dulbecco's Modified Eagle Medium), is improved on the basis of MEM Medium and has been widely used in the culture of various cells. DMEM high sugar medium is widely used in cells with fast growth and low adhesion. It can be used for the culture of a variety of mammalian primary cells, such as primary fibroblasts, nerve cells, glial cells, HUVEC, smooth muscle cells, etc. It can also be used for the culture of some cell lines, such as Hela, 293, COS-7 and PC-12, etc.

 

This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients needed for cell culture, without protein or growth factors, and needs to be used with 5-10% serum or no serum additives according to the type of cell.

 

This product is filtered and sterilized by 0.1 μm membrane, pH 7.0-7.4, containing 25 mM D-glucose, added 4.0 mM GLUTamax-I, contains phenolic red indicator, contains no sodium pyruvate, contains 25 mM HEPES buffer system.

 

Glutamax-i (L-alanyl-L-glutamine) is a cell culture additive that can replace L-glutamine in cell media. The substance is very stable in aqueous solution and does not spontaneously degrade. Instead, it is slowly degraded by a peptidase secreted by cells to release glutamine, which is then taken up by cells.

 

HEPES is an excellent biological buffer, which has no toxic effect on cells, and the medium added with HEPES can maintain a constant pH range for a long time.

 

Storage and transportation

 

Normal temperature transportation, 2-8℃ storage, 12 months of validity.

 

Matters needing attention

 

1. The product is filtered and sterilized, and sterile operation should be paid attention to when using to avoid contamination;

 

2. In order to maintain the best use effect, do not freeze and thaw treatment.

 

This product is only for scientific research purposes, not for clinical diagnosis!