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Position： Australian fetal bovine serum
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Product number: DIB-52A-500ml DIB-52B-50ml
Heat inactivation
Precautions and Disclaimer
Australian fetal bovine serum
Product number: DIB-52A-500ml DIB-52B-50ml
Product Description
Serum is the blood component that can be obtained after coagulation and by removing cellular components.Besides serum proteins it contains, e.g. growth factors, amino acids and hormones. This comprehensive mixmakes serum one of the most important supplements, supporting cells to grow and proliferate in vitro cell cul-ture. Of special interest is the fetal bovine serum (FBS). It is especially rich in growth factors and is particularly low inantibodies, which may influence the cell culture work.
| Product | Origin | Treatment | Volume | Cat. No. |
| Fetal Bovine Serum | Origin Australia | - | 50ml | DIB-52B |
| 500ML | DIB-52A | |||
| Heat Inactivated | 50ml | DIB-HI-52B | ||
| 500ML | DIB-HI-52A |
Additional Tests & Treatments
-Tested according to 9CFR
-Gamma Irradiation
Product Specifications
| pH | 6.8-8.2 |
| Osmolality | 280-340mOsm/kg |
| Endotoxin | As reported |
| Total Protein | 3.0-4.5g/dl |
| Albumin | As reported |
| Hemoglobin | ≤ 25 mg/dl |
| Mycoplasma | Not detected |
| Virus tested for | PI-3, BVDV, BVDV-AB, BHV-I |
| Sterility | Tested |
| Storage | Store at ≤-15°C. |
Heat inactivation
Heat inactivation will inactivate the complement system, antibodies and other active enzymes. It has to be done
in a carefully controlled process in order to avoid damaging the cell growth promoting properties of the serum
and reducing the formation of unwanted precipitates.The process involves heating the serum in a shaking water bath at exactly +56°C for 30 minutes. The shaking
will help avoid the formation of protein and other forms of precipitates. After 30 minutes the serum is then
cooled back down to room temperature as quickly as possible to avoid excessive exposure to heat which can
damage e.g. growth factors and vitamins.
Gamma irradiation
Gamma irradiation
Data Invention Biotech has established process parameters and controls for maximum inactivation of contami-nants by an innovative gamma irradiation process in small sized boxes at 25 – 35kGy. When FBS bottles are arranged in a pallet during gamma irradiation, high irradiation doses (58 kGy) are necessary to irradiate the
centrally located bottles with the required does (>30 kGy). This and the associated higher temperatures mayaffect the serum quality. If the irradiation is performed in single boxes, the maximum does of irradiation is performed in single boxes, the maximum dose of irradiation does not exceed 38.7 kGy. Gentle irradiation as
used by Data Invention Biotech is less likely to affect final serum quality.
Quality control
Quality control
Only sera batches which pass our strict quality control are released for sale. Standard parameters which are
determined include pH, osmolality, content of protein, albumin, IgG and hemoglobin, endotoxin level, sterility,
mycoplasma detection and virus testing.
Precautions and Disclaimer
This product is for research use only. Please consult the Material Safety Data Sheet for information regarding
hazards and safe handling practices.